Blot out trait in PAP

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Aug 6th, 2022
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How to blot out trait in PAP

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Wash slides in deionized or distilled water for 5 min at room temperature. Destain slides in 0.5% HCl in 70% ethanol for 5-15 min at room temperature.
The pap stain has 3 solutions having 6 dyes. Solution 1 has Hematoxylin stain, a basic nuclear stain. Solution 2 has OG-6, an acidic cytoplasmic stain, has orange G with phosphotungstic acid (6 denotes the used concentration of phosphotungstic acid).
EA 65 reagent, Pap 3C reagent is an alcoholic solution of two acid dyes, Eosin Y and Light Green SF, with added Bismarck Brown dye and phosphotungstic acid (PTA).
Papanicolaou stain (also Papanicolaous stain and Pap stain) is a multichromatic (multicolored) cytological staining technique. The Papanicolaou stain is one of the most widely used stains in cytology, where it is used to aid pathologists in making a diagnosis.
The Papanicolaou stain is undoubtedly the most commonly used in cytology. It has three cytoplasmic dyes, orange G, eosin Y, and light blue. It can stain cells blue or blue-green (basophilia, cyanophilia), pink (acidophilia, eosinophilia), orange (orangeophilia), or indeterminate (gray-blue).
The first staining solution contains haematoxylin which stains cell nuclei. Papanicolaou used Harriss hematoxylin in all three formulations of the stain he published. The second staining solution (designated OG-6), contains Orange G in 95% ethyl alcohol with a small amount of phosphotungstic acid.
Hydration is the factor that has the most influence on the transparency of the cytoplasm during the Papanicolaou staining procedure. During the staining process, the level of hydration affects how the cytoplasm takes up the stains and can result in variable transparency in the stained cells.
PAP bluing solution is a variant of Scotts solution and is used specifically in Papanicolaou staining (Pap staining) for cytological smears. It is used to bluish the hematoxylin in the stained nuclei to improve contrast and visibility, which facilitates the interpretation and analysis of cytology specimens.

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