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in this video youamp;#39;ll learn how to transfer proteins from polyacrylamide gels to membranes using the Invitrogen power blotter with our pre-cut membranes and filters in power bladder one-step transfer buffer the power bladder one-step transfer buffer is an optimized high ionic strength buffer that facilitates rapid transfer conditions when combined with the high current conditions of the power bladder system filter paper soaked in the one-step transfer buffer act as an ion reservoir the system can also be used for standard semi-dried transfer protocols that use traditional buffers in the power bladder system the cathode and anode are plates that are fixed to the top and bottom of the cassette frame and the transfer stack is placed in between them during the transfer process the power bladder system has two different cassette sizes a standard size for transferring one midi gel or up to two mini gels or the XL cassette for transferring up to 2 midi gels or up to 4 mini gels at one