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hello and welcome to todayamp;#39;s video tutorials for biochemistry practical todayamp;#39;s practical will be centered on how to stain an sdsp gel using the silver staining technique as we said in the video of the commercial blue staining the proteins that were separated on sds page cannot be visualized with naked eyes staining dyes are then used to visualize the proteins on sdsp gels last week we used the brilliant blue dye solution to stain but today we will use the silver stain solution silver stain is also used as one of the most sensitive calorimetric methods for detecting proteins the silver ions are reduced to metallic silver which deposits on the proteins giving it a brown black color the silver staining is performed like developing a photographic film and it is more sensitive than the kumasi blue staining and so the goals of todayamp;#39;s practical are to stain an sdsp gel with the silver stain and also to estimate the molecular weight of a protein using
