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so weamp;#39;re going to pretend that itamp;#39;s been 10 minutes it really hasnamp;#39;t but weamp;#39;re going to go ahead and Dain our DOT lot now now if you take a close look you can actually already see the faint outline of some proteins we had a spot there a spot there a spot there and a spot there and you can just barely kind of make that out already so those are going to clear up a lot when we when we do our Dain so and these since we did it very early in the procedure we we washed our resin with a a smaller volume than usual because weamp;#39;re doing a co- purification we may see a little bit more protein than expected and weamp;#39;re hoping that this one is mostly clear so Iamp;#39;m just going to go ahead and start to pour on some of our D stain I try to pour it on from a little bit higher blast it so you can see those are developing a little bit and now you see that a picture is really starting to take place here so you can see see very clearly we have two or four
