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[Music] were going to teach you how to set up and run your training kit on Jess start by preparing your standard pack reagents add 40 micro liters of water to the dtt tube mix by pipetting up and down to the pink florescent master mix tube add 20 microliters of 10x sample buffer and 20 microliters of the prepared dtt solution mix by pipetting up and down to prepare the biotin elated ladder add 20 microliters of deionized water to the blue tube and mix by pipetting up and down next prepare the Gila cell lysate prepare 0.1 X sample buffer by adding 1.5 microliters of 10x sample buffer and 140 8.5 microliters of deionized water mix by pipetting up and down then add 18 microliters of HeLa cell lysate with 72 microliters of 0.1 X sample buffer finally combine 20 microliters of prepared 5x florescent master mix with 80 microliters of your diluted HeLa lysate sample mix by pipetting up and down vortex and denature your samples at 95 degrees Celsius for five minutes vortex spin and then stor
