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but weamp;#39;ve done this morning was to run a double set of gels I have a little bit of water milic you water and a casserole dish Iamp;#39;m going to float four pieces of nitro cellulous on the water and when I run my Western transfer I actually like to have two pieces of nitro cellulous / gel so thatamp;#39;s why Iamp;#39;m hydrating four pieces now weamp;#39;re going to pour off the water and add our transfer buffer about a half inch of transfer buffer and weamp;#39;re going to wet one filter paper and put one nitro cellulous over the filter and then the second nitro cellulous right on top of that so now letamp;#39;s take our gels out we want to loosen it from its cassette slide it right up the straight edge of a spatula usually works to sort of pry the dil bottom open Iamp;#39;m going to go ahead and cut off the tops of the wells just scrape them off that weamp;#39;re going from one to ten from left to right we actually want to sit down on the micro cellulose in that sam
