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hello and welcome back to AA biotech Iamp;#39;m AA and today weamp;#39;re diving into the crucial first step of any successful Western blood experiment sample preparation proper preparation is essential for accurate and reproducible results letamp;#39;s get started firstly letamp;#39;s look at the importance of sample preparation why is sample preparation so important well it directly affects the quality of your results poor preparation can lead to degraded proteins inconsistent loading and ultimately unreliable data by following these steps you ensure your samples are ready for the best possible outcomes so letamp;#39;s talk about sample collection and storage whether youamp;#39;re using tissue or cells itamp;#39;s crucial to work quickly and keep everything on Ice to prevent protein degradation after collection store your samples at - 80 C until youamp;#39;re ready to proceed now we looking at cell liis the goal here is to break open the cells to release the proteins