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in this step iamp;#39;m going to be preparing my cell lysate samples so weamp;#39;re going to denature them in order to run them um in the sds page gel so for that i basically use the bio-rad 4x lamellae sample buffer so the first thing that we need to do when we look at the protocol so very important that you have the protocol for the 4x and not the 2x so you need to make sure that you have the appropriate lamellae buffer so for this the first thing that iamp;#39;m going to do is prepare it so weamp;#39;re going to use 900 microliters of the forex lamellae buffer and to that weamp;#39;re going to add 100 microliters of beta mechata ethanol so i already have my tube here and iamp;#39;m going to go ahead and prepare that we have our nine hundred and to that weamp;#39;re going to go ahead and add the 100 microliters of beta mecha ethanol so i already have my pipette ready if youamp;#39;re sensitive to the smell of vitamin catholic ethanol you can go ahead and do this in the chemi