No matter how complex and hard to change your documents are, DocHub delivers a straightforward way to change them. You can alter any element in your WRF without effort. Whether you need to fine-tune a single element or the whole form, you can rely on our robust solution for quick and quality outcomes.
Moreover, it makes certain that the final document is always ready to use so that you’ll be able to get on with your projects without any delays. Our all-purpose set of tools also comes with sophisticated productivity features and a collection of templates, enabling you to make best use of your workflows without the need of wasting time on recurring operations. On top of that, you can gain access to your papers from any device and integrate DocHub with other solutions.
DocHub can handle any of your form management operations. With an abundance of tools, you can create and export paperwork however you choose. Everything you export to DocHub’s editor will be saved safely as much time as you need, with strict protection and information safety frameworks in place.
Check DocHub today and make managing your paperwork easier!
After Western blot transfer is completed, the membrane undergoes a series of incubation and wash steps to prepare the membrane and probe it for your target protein. The first step, called blocking, coats the membrane surface with a protein or other molecule that is non-reactive to the antibody that will be used to probe for the target protein, or antigen. Nitrocellulose and PVDF membranes have a high protein-binding affinity, which is essential for successful Western blot analysis. The blocking step ensures that the antibody binds specifically to the antigen rather than non-specifically to the membrane surface, which can result in a high background signal that prevents detection of your protein of interest. The membrane is blocked with a buffer that is often comprised of Tris- or phosphate-buffered saline with Tween-20 detergent and up to 5% of a non-reactive protein such as bovine serum albumin, non-fat milk, gelatin or normal serum. The exact formulation and incubation period depends
