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this is USMLE QA USMLE material presented in a question and answer format structured similar to the first date for the USMLE step one book question what is the first step in the southern block procedure answer the first step in the southern block procedure is to enzymatically cleave the DNA sample into smaller pieces question how are the smaller pieces of DNA separated during the southern block procedure answer the smaller pieces of DNA are separated on a gel by electrophoresis question what happens to the separated pieces of DNA after they are separated on the gel answer after they are separated on the gel the DNA pieces are transferred to a membrane question what is the membrane exposed to after the separated pieces of DNA are transferred to it answer the membrane is exposed to a labeled DNA probe that anneals to its complementary strand question what is the resulting labeled piece of DNA after the membrane is exposed to the label DNA probe answer the resulting labeled piece of DNA i
