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welcome to the gel analysis dot blot analysis tutorial for the unscanned gel software this tutorial will show you how to use the dot blot analysis mode of the un-- scan at gel software to quantify dot blot images when the software is opened a startup screen will appear and a popup menu will allow you to analyze a gel this option can also be selected from the main analyze gel menu or the analyze toolbar the dot blot image can be in a variety of image formats including TIFF JPEG bitmap gif and PNG formats the image can come from a scanner digital camera imager internet or other image source for this tutorial we will choose a sample JPEG image from a scanner there are several gel analysis modes available including Lane analysis segment analysis and dot blot analysis these modes are explained further by clicking the question mark button associated with each mode for this tutorial we will choose the dot blot analysis mode multiple gel analysis options will be presented and you can choose th