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the iblot western detection kit allows you to do an entire western in just 30 minutes or less the blocking step the primary antibody incubation and the secondary antibody incubations are performed in the iblot device the same device that you use for protein transfer and at the end of the secondary antibody step you simply do some quick washes outside the device and then develop your blood just like you would today the iplot western detection kit allows you to get your western results in less than 30 minutes you begin by diluting your water solution this is a 16x wash solution you will use the antibody diluent solution to make your antibody solutions and this also serves as the blocking solution so we begin by making the antibody solutions and the wash solutions we place one of the matrices on the plastic sheet provided in the kit you pipet the antibody diluent solution for the blocking step onto the matrix itamp;#39;s a very absorbent material so it will spread the solution quite nice
