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hi everyone so in this video iamp;#39;m going to show the other method for quantifying your band so youamp;#39;ve electrophoresed the proteins down a gel youamp;#39;ve placed the gel on a nitrocellulose or pvdf membrane and transferred the protein from the gel to the membrane so that you can probe it with a specific antibody. you probe with a specific antibody or two and the antibody is a protein so thereamp;#39;s no way to physically visualize it, to detect it so these antibodies are often coupled to other reagents to allow you to be able to detect it they may be coupled to a fluorophore, a fluorescent molecule so you can detect it using laser or it may be coupled to enzymes such as alkaline phosphatase or horseradish peroxidase and in this case by supplying a substrate for the enzyme a reaction will happen that you can visualize you can detect. in the past radioisotopes were also extensively used. so once youamp;#39;ve detected your antibody or your
