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if you have gone through our previous video lectures on this channel following concepts should be clear to you these are primary and secondary antibodies reporter enzymes immunoassays such as Eliza and sds-page I have covered basic principle of SDS page and its application in our second channel please go through it once if this technique is new to you link is given in the description of this video Western blotting is a widely used technique for the detection and analysis of proteins blot refers to the membrane on which biological molecules such as proteins and nucleic acids are immobilized the process of transferring macromolecules from a gel to a membrane followed by their detection on the membrane is known as blotting when the macromolecule involved is DNA the technique is known as southern blotting southern is the last name of the scientist who first blotted DNA sir Edwin Melo southern by analogy blotting involving RNA is known as northern blotting and for protein this technique is
