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this is a quick tutorial in how to do restriction mapping and what i mean by that is when you have a plasmid that youamp;#39;ve digested with some restriction enzymes how to interpret the bands and draw a map of the plasmid from those bands so here we have a hypothetical gel what you have is the wells at the top of the gel youamp;#39;ve got five different lanes lane one is your dna ladder and weamp;#39;ve marked um all of the fragments on the ladder 700 base pairs 500 base pairs etc lane 2 is the undigested plasmid and itamp;#39;s 700 base pairs lane 3 is the plasmid digested with eco r5 lane 4 is the plasmid digested with bam h1 and lane 5 is the plasmid digested with both eco r5 and bam h1 and so the question is how do you interpret this data and get to a map of the plasmid that has the locations or the relative locations of the eco r5 restriction sites and the bam h1 restriction sites all right i have redrawn the gel and labeled each of the lanes so letamp;#39;s see how we can
