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hello and welcome to the introductory video for the Western blot in this short introduction Iamp;#39;ll show you what is it and what is it used for so to start off with what is a western blot essentially what weamp;#39;re doing we are blotting proteins onto a nitrocellulose membrane to we read on a reader to see whether our protein of interest is there in our sample so after blotting our proteins onto the membrane we then apply a layer of primary antibodies these antibodies are monoclonal Mouse antibodies so these are antibodies which are GR in a mouth which will Target our specific protein of Interest we can then apply secondary antibodies so these are rabbit antimouse antibodies which will only bind to the primary antibodies and attached to these secondary antibodies they have an enzyme called horseradish peroxidase attached to it or HP for short and what this will do this will cataliz a reaction of luminol into a compound called three Amino phalate which is a fluorescent product w
