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welcome to unboxing the Western blot workflow we will begin by running our protein samples on new page boosters gels using the Excel Sherlock gel apparatus we will use the Novick sharp pre-stained standard to estimate molecular weight once our samples are run will transfer them to a membrane using the I blot dry plotting system once the proteins are transferred to the membrane we will move on to do Western processing and we will have three options we could either use the ipod again and use the i blog Western detection kids or we could use the bench for 4,100 automated Western processing stations or finally we could do it manually using the Western breeze kids