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hi everyone the focus of todayamp;#39;s tutorial is just going to be uh to look how we can pronounce densitometry now density is a very powerful technique and it basically allows us to quantify this sort of band so of course we can see that this band appears a bit darker than this one which suggests we potentially have more material in here assuming we loaded the same amount of protein but how many folds more of proteins do we have here compared to here so this is when we need to perform some sort of quantitative analysis on this gel now when you take a picture of your gel of course you use some kind of a machine thatamp;#39;s associated with a computer and typically the software in that computer will be able to perform the densitometry for you what we are going to do today is to perform this densitometry manually to kind of understand whatamp;#39;s happening uh behind the blinds whatamp;#39;s happening behind the screens how the algorithms within those softwares actually work okay
