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(twinkling intro music) - [Instructor] Western blotting, also called immunoblotting, is a widely-used technique to monitor protein expression in a cell or tissue extract based upon antibody binding to a specific protein of interest. Antibodies used in Western blotting can be specific to total protein, or to a post-translational modification of a protein, such as sites of phosphorylation, acetylation, methylation, ubiquitylation. - At Cell Signaling Technology, we perform Western blots daily to validate our new and existing antibodies. Here, we provide a complete protocol for the technique based upon a decade of our experience. We also provide a list of commonly used reagents that are used by CST scientists and work optimally with our antibodies. Using additional directions found on your datasheet, you can simply replicate what we are doing here, and get the expected results in the shortest amount of time. We will also discuss the critical steps in Western blotting and how protocol chan
