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hello and welcome back to bioinformatics series of videos on explore bio with Dr Abhishek a particular protein in different organisms has different sequences one common way to search similar or homologous proteins is by performing blast homology search I have made a complete tutorial on how to perform blast locally on your computer its link is provided in the description below Hammer is a tool used to identify the proteins or conserve profiles of protein families based on protein sequence or alignments with hammer you can use protein query or domain profiles to identify homologous proteins or conserve domains in a reference database there are two modes to perform hammer in this tutorial I will show you how to use the online version of hammer in the web version you see four options the first is p hammer here you can check the homology of a query protein sequence with entire protein database reference of your interest just copy a protein sequence to the search window next select referenc