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in this video we will show you how to assemble the transfer stack for western transfer using a transplot turbo transfer system the transplot turbo system is compatible with standard transfer materials however today we will demonstrate the use of transplot turbo transfer packs for rapid transfer first open the transfer pack by peeling back the foil to reveal pre-soaked transfer stacks take the bottom stack containing the membrane and place it inside the transfer cassette with the membrane on top wet a blot roller with buffer then roll the stack to remove any trapped air bubbles next take your gel cut off the wells with a straight edge and place it on top of the membrane roll the stack again next place the top stack over the gel and roll again to remove air bubbles place the cassette cover over the top of the stack press firmly and turn the green knob to lock the cover in place insert the cassette into the instrument then access the turbo protocol for a 7 minute rapid transfer if