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what are you waiting for do it already arenamp;#39;t you forgetting something what about a loading control shut up mr. golden black hey watch your tone a loading control is definitely necessary if you want to be sure changes on your blotter real oh we already have to do this 20 times one more work just because blockhead love us says so stop name calling you you messy membrane okay that gap th it is wait what why cap th thatamp;#39;s what everybody uses weamp;#39;ve never had any problems before maybe we should make sure whatever we use has stable expression for our cell types and treatments we also need to consider itamp;#39;s good enough nobody needs to know about proteins in membranes and buffers validated itamp;#39;s worth it how confident are you in your Western blots watch our normalization webinar to find out how to reduce variability and error
