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asalam alikum students today we are going to conduct a very important experiment itamp;#39;s about protein identification the title of the experiment is Western blotting you know that identification of prot have importance not only in Diagnostics but also in basic research the basic and fundamental principle of vestern blotting is very simple you identify protein of your interest through its interaction with an antibody so you design an antibody which is specific for binding with your protein in this way if that antibody binds with your protein you will get the signal which will indicate that your protein is present and if the antibody is not binding because there is no such protein in your sample then it will mean that your protein is not there also this experiment tells you about the down regulation your over regulation thatamp;#39;s overexpression or under expression of your protein of Interest so if your protein is being produced in a very lower amount the signal produced
