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[Music] welcome back friends welcome to another video lecture from shomus biology and in this video tutorial we will be talking about western blotting we have been talking about different types of blotting techniques lately weve talked about southern blotting northern blotting and this time well be talking about the western blotting blotting is a technique of detecting macromolecules if you are trying to detect dna we use sound blotting if you want to detect rna we use northern blotting and if we want to detect proteins we use western blotting now northern blotting and southern blotting has very very similar techniques which uses mostly same type of techniques and setup while western blotting is little different compared to them in western blotting it is very very sensitive reaction technique that can even detect nanograms of protein molecules in the mixture of other proteins now there are different ways of detecting a target protein from a mixture we can use chromatin immunoprecipi