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NARRATOR: This is Larry. Hes a grad student in the biology department at MIT, and hes going to show us how to do a western blot from beginning to end. So Larry has six different samples. Theyre each mammalian cells that have been engineered to over-express a different human protein. Larrys removing the media, and then hes going to wash them to make sure all the medias gone before adding lysis buffer. Larry is preparing whole cell lysate, which includes all the proteins inside the cell, and then he scrapes to physically remove the cells from the petri dish. LARRY: So this is basically, once you put the lysis buffer and scrape, all of the cells are essentially popped open. The membranes are gone, and clear off the plate from the scraping. NARRATOR: So now all of Larrys sample are in Eppendorf tubes with a blue loading dye added to them to help visualize them. Hes going to load them into a precast polyacrylamide gel. And the buffer that hes going to add to the box is an SDS bu
