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in this video you will learn how to perform a Western blot a Western blot can be used to identify specific proteins in a sample and provide information about the protein size and relative abundance in the sample first fill a tray with blotting buffer youll be using this buffer to equilibrate your gel prior to starting the Western blot next remove the gel from the gel cassette using the opening key line up the arrows on the opening lever with the four arrows on the cassette to open the cassette you after trimming the top and bottom of the gel with a straight edge it collaborate the gel in the tray with blotting buffer for 15 minutes on a rocking platform pre-soaked fiber paths and blotting buffer so that they are thoroughly soaked to make a blotting sandwich obtain a container large enough to fit the gel holder and add enough blotting buffer until the container is filled approximately one centimeter deep place the gel holder cassette in the container with a black side down and immerse
