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the binic acid assay or commonly called the BCA assay is used to determine protein concentration by measuring the amount of reduced cus that is generated by Keating copper to proteins in the sample the cion then reacts with BCA forming a purple water soluble complex the thermoscientific Pierce BCA protein aay kit includes all reagents needed to accurately determine protein concentration in a sample including purified bovine serum alumin standard in single use 1ml ampules for consistent standard curve generation the kit includes protocols for both microplate and test tube based assays however we will just use microplates in this example after diluting the BSA standard prepare the BCA working reagent by combining reagents A and B at a 50 to1 ratio and pipet 25 micr of each standard or sample into a microp plate well followed by 200 microl of the BCA working reagent gently mix the components for 30 seconds and then incubate the plate at 37 for 30 minutes after cooling the plate at room t
