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hello and welcome everyone to our immunoprecipitation webinar today Iamp;#39;d like to give you a nice talk about immuno precipitation procedures pitfalls and protocol tips so as an overview to my talk I first like to give an introduction to immuno precipitation what it is and how it works I then like to go over a detailed protocol regarding immuno precipitation analysis via Western blot then go over some practical examples of how this technique is used discuss the use of tags and fusion proteins and finally the part youamp;#39;re all waiting for is the troubleshooting tip section so letamp;#39;s first begin with an introduction so what is immuno precipitation IP is a method that enables the isolation of proteins or protein complex from a biological sample there are three main steps involved the first is the incubation of the sample with the antibody against your protein of interest then this antibody protein complex is separated from the remainder of the sample isolated and then an
