Blot mark in ASC

Utilize this fast walkthrough to blot mark in ASC quickly

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blot mark in ASC by reading these steps:

Register your DocHub account or sign in if you already have one.
Click the Add New button to upload or transfer your ASC into the editor. You can also utilize the tools available to tweak the text and customize the layout.
Choose the option to blot mark in ASC from the menu bar and use it to the form.
Check your form again to make sure you haven’t overlooked any mistakes or typos. When you finish, hit DONE.
You can then share your file with others or send it out using your preferred way.

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How to blot mark in ASC

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What activates the NLRP3 inflammasome?

K+ efflux. Early studies have shown that K+ efflux is a common phenomenon that activates the NLRP3 inflammasome. NLRP3 agonists first trigger cell membrane permeation of K+ and Na+, which in turn activates the NLRP3 inflammasome.

What are NLRP3 ASC specks an experimental progress of 22 years of inflammasome research?

Speck assembly is the hallmark of NLRP3 inflammasome activation. The 1m structure comprising of NLRP3 and ASC is the first observable phenotype of NLRP3 activation. While the common consensus is that the specks are the site of inflammasome activity, no direct experimental evidence exists to support this notion.

What is the full form of ASC protein?

Results suggest that apoptosis-associated speck-like protein (ASC) can function as an adaptor molecule for Bax and regulate a p53-Bax mitochondrial pathway of apoptosis. Methylation-mediated silencing of TMS1/ASC confers a survival advantage to ovarian tumor cells.

What is apoptosis associated speck-like protein ASC?

PYCARD, often referred to as ASC (Apoptosis-associated speck-like protein containing a CARD), is a protein that in humans is encoded by the PYCARD gene. It is localized mainly in the nucleus of monocytes and macrophages.

What is the size of ASC specks?

ASC specks can be observed as they docHub a size of around 1 m and in most cells only one speck forms upon inflammasome activation. Hence, ASC speck formation can be used as a simple upstream readout for inflammasome activation.

What is ASC in inflammation?

Apoptosis-associated speck-like protein containing a CARD (ASC) has a pivotal role in inflammasome assembly and activation. While ASC function has been primarily implicated in innate immune cells, its contribution to lymphocyte biology is unclear.

What is the role of ASC in the inflammasome?

Upon inflammasome activation, ASC polymerizes into a large speck-like structure, which functions as a scaffold for caspase-1 cleavage and activation, a process which also requires the intracellular redistribution of ASC [11,12].

What are ASC specks?

ASC specks are micrometer-sized structures formed by the inflammasome adaptor protein ASC (apoptosis-associated speck-like protein containing a CARD). ASC specks are characteristic of inflammasome activation. The inflammasome adapter ASC is localized mainly in the nucleus of macrophages and monocytes.

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