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welcome to this episode of mentors at your bench side the podcast giving you advice tips and tools for getting the most out of your research Iamp;#39;m Laura grassy and today Iamp;#39;ll be talking to you about how ponzoess staining can help troubleshoot your Western block problems you know how many problems can crop up if you routinely perform Western blotting Ponce staining can help identify issues early on saving you time and effort in incubating and developing bad plots in this episode weamp;#39;ll cover how pontus Works how to perform staining and give you an overview of how to interpret what the stain means when troubleshooting your failed blots so first what is ponzo s and how does it work ponzo s is a die that binds to proteins on pvdf or nitrous cellulose membranes producing red bands where there is protein unlike kamasi blue the other standard dye for visualizing proteins after SDS page ponzo s is reversible and is therefore compatible with most Downstream applications the