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sometimes in a molecular biology lab youamp;#39;ll hear people that you think are lost and using a compass to try to find their direction this is a blotting compass because a lot of the blots get their names from cardinal directions so these are blots in molecular biology well starting first with a southern blot a southern blot is a sensitive method for detection of a dna sequence within a mixture the dna is first cleaved or cut with restriction enzymes to produce fragments that can be separated by gel electrophoresis after electrophoresis dna fragments are transferred to a nitrocellulose membrane by capillary action creating a replica of the original gel the membrane is incubated with a labeled probe which binds to and detects fragments of interest so we have our dna we have our gel run it through we have this transfer step we have this blotting step and ultimately what weamp;#39;re left with is what looks like lines in a column specifically this is looking at dna and this is why it
