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MilliporeSigma and Seeding Labs present: Western Blotting: How to Optimize Your Signal to Noise Hi everybody. My name is Jun Park. Today Iamp;#39;d like to share with you some of the simple steps that you can use to optimize your signal to noise with your Western blots. So let me show you an example. Say Iamp;#39;m not really sure whatamp;#39;s going on lately, but Iamp;#39;m getting a bad blot that looks like this. So the blot is actually not clean at all and you can see that itamp;#39;s actually looking pretty dirty. Another thing that you will notice that in addition to this background, there is a lot of other bands that really should not be there in the first place. I know this is my target protein band because of the molecular weight but if you look at it, thereamp;#39;s a lot of other bands that are present. So when you get a blot like that, or when I get a blot like that, what can I do to troubleshoot a situation like this? Now if you have done a lot of Western blotting, y