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western blot is a fundamental biochemistry technique we can use to detect the presence and relative amounts of proteins that weamp;#39;re interested in so basically how it works is we take a protein we take a sample that contains a bunch of proteins maybe you break open a cell and take that lysate and maybe do it from cells under different conditions like with or without a drug or different cell types that you want to compare the expression of this protein in then what youamp;#39;re going to do is you separate all the proteins in that sample using an sds page gel so basically you denature or unfold the protein coded in this negatively charged sds detergent send it traveling through this gel and then the proteins are going to separate by size and so you have a bunch of proteins like separated by size through this gel because the longer ones are going to travel slower because they get stuck more in the gel so itamp;#39;s going to take them longer and you turn it off and if you were ju