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hi everyone in this video weamp;#39;ll be talking about Western blotting and its implication on mad cow disease letamp;#39;s recap what western blotting is Western blotting is a lab technique used for finding specific proteins in a given sample such as blood or tissue to do this first a sample is prepared that contains proteins of Interest next gel electrophoresis is used which is a technique using charge to separate the proteins it is separated ing to its weight measured in KDA unit when voltage is applied smaller proteins move up faster in the SDS page which is the gel being used next the proteins from the gel are transferred to solid membrane and antibodies are added to make proteins of Interest detectable antibodies basically bind to the Target protein and the choice of antibody depends on the type of antigen needed to be detected after that blocking is done which is to prevent antibodies from binding to the Protein thatamp;#39;s not of Interest lastly proteins are detec
