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hello and welcome to my channel lab easy so in my previous video i have discussed about the procedure the step-by-step procedure of performing and western blot and today we i will be discussing what went wrong in your western blood so in this video we will discuss the problems that may arise in your resting plot and which are usually very common so getting a perfect plot in the first time can be very tricky and there might arise several problems so we will discuss about them and we will discuss the probable solutions by which you can improve your plot so letamp;#39;s begin so i will go step by step the first step is separation as i have discussed in my previous video also please check out the link below and see my previous video here so in the step of separation that is our protein samples are being separated ing to their molecular weight so what may happen is that if your protein is a very high molecular weight it may not be very well resolved in your gel so what may happen is that i