Blot issue in ASC

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How to blot issue in ASC

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hello mark hello everybody out there welcome to the todayamp;#39;s webinar about Western plotting and the troubles you can have with this method first of all I want to give you an overview about the Western plot feature and then I will show you possible problems can be revealed by the detection system so what can go wrong on how can you solve the problems and at the end you will see the way how to get a good Western plot so letamp;#39;s start with the Western plotting workflow so you can see itamp;#39;s a quite complex method with different steps so why do you perform a Western plotting analyzes so first of all you want to confirm the presence of a target protein in a special cell acid or tissue preparation and you want to obtain FEMA quantitative information so if a certain target protein is regulated in a special way by a special substances and these things so hmm since we have technical problems okay here we are first of all thatamp;#39;s the sample preparations and this is at t

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What is apoptosis associated speck-like protein ASC?

PYCARD, often referred to as ASC (Apoptosis-associated speck-like protein containing a CARD), is a protein that in humans is encoded by the PYCARD gene. It is localized mainly in the nucleus of monocytes and macrophages.

What is the function of ASC?

The main functions of ASC are: Screening of advertisements, with or without advertising claims. Hearing of disputes on advertising content. Resolution of disputes on advertisements arising from procedures of the ASC.

What is the role of ASC in the inflammasome?

Upon inflammasome activation, ASC polymerizes into a large speck-like structure, which functions as a scaffold for caspase-1 cleavage and activation, a process which also requires the intracellular redistribution of ASC [11,12].

What is the function of ASC in Inflammasome?

For the activation of inflammasomes, ASC can form large oligomeric filaments to facilitate the recruitment of procaspases. Sborgi et al. applied cryo‐EM and NMR and determined that ASC assembles to long filaments in response to pathogen invasion and thus amplifies the inflammatory signal.

What is ASC in pyroptosis?

Oligomerization of sensor proteins further recruits CARD domain-containing adaptor protein apoptosis associated speck like proteins (ASC). ASC brings in caspase-1 to inflammasomes for oligomerization, which leads to caspase-1 activation through the auto-cleavage.

What is the function of the ASC gene?

Results suggest that apoptosis-associated speck-like protein (ASC) can function as an adaptor molecule for Bax and regulate a p53-Bax mitochondrial pathway of apoptosis. Methylation-mediated silencing of TMS1/ASC confers a survival advantage to ovarian tumor cells.

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