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in this video we are going to cover the core content you need to know about blotting for the mcat since the mcat cares most about the interpretation of bots we will spend most of our time focused on that and will only cover the method of preparing a blot briefly in general a blot has three major steps running a sample on a gel to separate it by size transferring the samples from the gel via blotting basically pasting a piece of special paper on top of the gel to absorb the samples and finally visualizing the sample using an antibody or a couple of antibodies designed to detect a specific molecule or set of molecules for example lets say you wanted to know how much adenosine diphosphate and adenosine triphosphate was present in three different cell line cytosol you would first extract the cytosol from those cells run them on a gel transfer that gel to a blotting paper then expose the blotting paper to a marker designed to detect both atp and adp then look at the plot since the samples
