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hey come run Western blots with me now youamp;#39;re wondering whatamp;#39;s a western by now is a molecular technique use to detect a specific protein and basically like a mixture of proteins like these now our first step is to determine the concentration we typically do it by a BCA acid now once we determine our concentration we can prep the samples letamp;#39;s talk proteins now proteins are typically in the quinary structure one of our main goals here is to put them back in the primary structure this will be done by heating them at 95 Celsius for about 5 minutes and adding a loading Dy which contains 10% SDS this step will get them back in their primary structure as well as coat the protein with a uniform negative charge we can finally load our samples our first thing will be our protein molecular weight marker this marker will be used as a reference it will be used to identify your expected band based on the molecular weight the rest of your samples can be loaded in your Desir
