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- How do I choose positive loading controls for my western blot? Iamp;#39;m Alex, a product scientist at Cell Signaling Technology, and this is CST Tech Tips. (piano music) When we talk about positive controls with customers, weamp;#39;re generally talking about a lysate. Itamp;#39;ll be from a known and published sample that expresses your protein of interest. Often customers might think of a positive control as a loading control like GAPDH, rather than a side-by-side lysate comparison of the target. So letamp;#39;s distinguish between these two approaches. What does a positive lysate control tell you compared to a loading control? When loading relatively equal protein amounts, the lysate allows you to compare expression levels between different cell types or experimental samples. Your protein of interest may be less abundant in your cell model compared to a loading control protein, which is typically highly abundant. So the control lysate gives you a more biologically relevant id
