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in this tutorial we will learn how to quantify western blood bands using image j software to quantify the intensities of western blot bands log on to the website as shown in the screen below click on download and select the software that suits your operating system after the download has been completed extract the file and find the image j icon inside the folder for your convenience the image j icon can be exported to the desktop this tutorial let us import a western block image by drag and drop method onto image j in this example the block image on the upper side in red shows the fibulin 1 pans consisting of sample 1 and sample 2. on the lower side of the plot in green the bands are of a housekeeping gene cap d8 to perform a densitometric analysis of the bands of our interest first the image has to be converted into an 8-bit or a 16-bit image the bands in the block can be made more visible by changing the intensities manually change the brightness and the contrast ing to your choice o
