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[Music] [Laughter] hi welcome to the channel which is about image processing image analysis and some general science chat i dont do gels and blots measuring western blots and so on um ive always been curious as to how the image analysis is done on these types of images and so i generally have a question for anybody whos watching this what is the best way to do it ive noticed that in mhj and fiji there is a gels plug-in but to me it seems a bit subject to interpretation and suppose most image analysis actually is but lets have a look at how its normally done using the gels plug-in and i want to consider whether or not using just simple thresholding and regions might be an alternative and im curious to know what people think about how you should measure a gel so as i said in the introduction the the channels about chat science chat and image analysis and image processing and in this particular video then id like us to have a chat about what is the best way to analyze a a gel or