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foreign ERS tides are metabolites that have several regulatory functions in eukaryotes including the control of gene expression protein trafficking signal transduction and cell development they perform this regulatory function by binding to proteins thereby changing protein conformation catalytic activity or protein tractions the identification of proteins that bind to non-stop phosphates or phosphonous diets is essential to understand how this metabolites perform the regulatory function this protocol has a simple workflow that sensitive non-radioactive lipsum free and uses regions they are commercially available in this method I finished chromatography with biotinylated non-sulfosphates of phosphinusitis is used to isolate intracting proteins that identified by Western blood or Mass spectrometry for bloodstream forms grow tbrus the eye cells to Mid log phase in hmi-9 media supplemented with 10 FPS at 37 degrees Celsius with 5 carbon dioxide keep the cell density between 800 00
