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we understand that the genome of each organism contains thousands of genes each gene or DNA sequence is transcribed into RNA and then RNA is translated into proteins that play various roles in the body of an organism suppose gene X is present in the genomic DNA of a mouse now we want to know how much active is this gene in different tissues of the mouse letamp;#39;s say how much active is gene X in the cells of the liver heart and intestines of the mouse by dumb active I mean at a given time how actively this gene is transcribed in biological vocabulary we want to study the level of gene expression in each case level of gene expression can be easily determined if we find out the amount of RNA transcribed from gene X in each tissue for this we need to do two things first isolate RNA from the different tissues of the mouse and second detect and quantify the specific RNA that is already transcribed from gene X the technique which will be suitable for this kind of gene expression and anal
