Blot identification in AFP

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Aug 6th, 2022
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How to blot identification in AFP

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hi everyone thanks for joining us for our Western blot webinar today weamp;#39;ll be going through the basic principles of the Western blot and Iamp;#39;ll show some examples of troubleshooting this technique remember that if you have any questions please submit them to the Qamp;amp;A panel on the right hand side of your screen and Iamp;#39;ll be available to answer some of them at the end of our presentation so what is a Western blot and how does it work itamp;#39;s a complicated technique but to summarize itamp;#39;s used to identify a specific protein of interest within a sample containing many proteins first the proteins are separated from each other ing to molecular weight using gel electrophoresis and then a specific antibody is used to identify the protein of interest so we can determine the molecular weight of the protein of interest by comparing the results to protein standards of known molecular weight and we can also determine the relative quantity of the protein of in

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AFP tumor marker normal range AFP is measured in nanograms per milliliter (ng/mL), with normal levels less than 20 ng/mL. Levels above 400 ng/mL may indicate a liver tumor or other cancer. Elevated levels of AFP may indicate cancer of the liver, ovaries or testicles, but that isnt always the case.
Generally, an AFP level between 0 ng/mL to 10 ng/ml or even up to 40 ng/mL can be normal for adults But normal levels vary, so talk with your healthcare provider about what your results mean for you. An extremely high level of AFP in your bloodgreater than 400 ng/mLcould be a sign of liver tumors.
An AFP tumor marker test may be used during the diagnosis and/or treatment of cancer of the liver, ovaries, or testicles that make high levels of AFP. It is used to: Help confirm or rule out a cancer diagnosis when used with other exams and tests. Predict how cancer may behave over time.
Alpha fetoprotein (AFP) is a protein produced by the liver and yolk sac of a fetus. AFP has no normal function in adults. The alpha fetoprotein test (AFP) is a blood test performed to measure, diagnose, or monitor fetal distress or fetal abnormalities. It can also detect some liver disorders and some cancers in adults.
Alpha-fetoprotein (AFP, -fetoprotein; also sometimes called alpha-1-fetoprotein, alpha-fetoglobulin, or alpha fetal protein) is a protein that in humans is encoded by the AFP gene.
The symptoms of AFP or AFM include: weakness and loss of muscle tone in the limbs. facial droop or weakness. difficulty moving the eyes.
Increased AFP - Levels higher than 400 ng/mL are considered diagnostic with appropriate imaging studies.
AFP is one of several tumor markers. Tumor markers are molecules in the blood that are higher when a person has certain cancers. AFP is found mainly in liver cancer and germ cell tumors of the testicles or ovaries, which are rare. These are found in the pineal gland in the brain.

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