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[MUSIC PLAYING] NICO WAGNER: Hi, Iamp;#39;m Nico and Iamp;#39;m a PhD student in the Molecules, Cells, and Organisms program at Harvard University. In this video, I will show you how to perform a Western blot. The purpose of a Western blot is to specifically look at a single protein and visualize it on a camera. The steps included in running a Western blot are a protein gel, which can be found in a different video. But using the product of this protein gel, the proteins themselves are transferred onto a membrane. A primary antibody is added to the membrane to specifically bind to your protein of interest. A secondary antibody binds the first antibody, and the secondary antibody usually contains some sort of fluorophore, which we are then able to see when placing everything into a camera system. To run a Western blot, the first step after running the protein gel is to assemble the Western sandwich. The Western sandwich consists of three sheets of Whatman paper, the gel, a nitrocellulo
