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NARRATOR: This is Larry. Heamp;#39;s a grad student in the biology department at MIT, and heamp;#39;s going to show us how to do a western blot from beginning to end. So Larry has six different samples. Theyamp;#39;re each mammalian cells that have been engineered to over-express a different human protein. Larryamp;#39;s removing the media, and then heamp;#39;s going to wash them to make sure all the mediaamp;#39;s gone before adding lysis buffer. Larry is preparing whole cell lysate, which includes all the proteins inside the cell, and then he scrapes to physically remove the cells from the petri dish. LARRY: So this is basically, once you put the lysis buffer and scrape, all of the cells are essentially popped open. The membranes are gone, and clear off the plate from the scraping. NARRATOR: So now all of Larryamp;#39;s sample are in Eppendorf tubes with a blue loading dye added to them to help visualize them. Heamp;#39;s going to load them into a precast polyacrylamide gel.
