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thank you hello everyone Iamp;#39;m A lab technician in the western blot detection Department of Wuhan boaster biological engineering co-limited Western blood experiments are often used to determine the expression of proteins quantitatively or qualitatively in tissues or cells the experimental steps are cumbersome and every detail in the experiments may have an impact on the results next I will share with you my practical experience over the years first letamp;#39;s take a look at what a complete Western blot experimental process looks like it can be roughly divided into the above 12 steps protein extraction protein quantification sample loading electrophoresis membrane transfer blocking primary antibody incubation secondary antibody incubation chemiluminescence detection this is our boaster product used in each step of the western blot experiment okay next I will use the booster antibody cyp-1b1 product number pb9546 as an example to demonstrate it for you st
