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in this video youamp;#39;ll learn how to transfer protein from polyacrylamide gels to membranes using the in vitro gene power bladder and select transfer stacks in vitro Jin power blotter select transfer stacks utilize a special application of the unique gel matrix technology developed for the in vitro jamp;#39;en AI blot to transfer stacks each stack contains the appropriate cathode and anode buffers in gel matrices so there is no need to prepare transfer buffer they also incorporate either PVDF or nitrocellulose membranes in the power bladder system the cathode and anode are plates that are fixed to the top and bottom of the cassette frame respectively and the transfer stack is placed in between the cassette during the transfer process the power blotter has two different cassette sizes a standard size for transferring one midi gel or up to two mini gels or the Excel cassette for transferring up to 2 midi gels or up to 4 mini gels at a time to begin the transfer process flip the pow