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How to blot guide in 600

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hi everyone iamp;#39;m kevin from apical scientific today iamp;#39;d like to introduce to you the new aso biosystem gel imager this is actually the top of the line system that we have from our imaging series and the one that you see here is actually the azo 600 first and foremost azure biosystem so our iso biosystem is actually a us-based company established since 2013 and they are specializing in manufacturing gel imaging system gel docks as well as western blot fluorescent imaging systems they are well more experienced in the market and they are considered experts in this field so we have six models in total okay ranging from 200 to 600 where by is differentiated by its application and of course its budget this particular model the azo 600 is capable of running your rgb fluorescence your nir your uv light blue light as well as your camera luminescent itamp;#39;s a fully compatible system which runs on windows os as you can see very flexible touchscreen panel and of course

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How to interpret western blotting results?

To know how to analyze western blot data, Look for the sizes of the bands. These will be represented by a number, either followed by kDa or preceded by p. This is the size of the protein which has been detected and is the scale on which the proteins are separated in a Western blot.

What is the range for western blotting?

Most laboratories use a range of 2040g of total protein for loading homogenates for western blotting. However, some labs use greater than 100g of total protein to detect lower abundance proteins.

What is the best concentration for western blot?

The minimum recommended concentration is 0.1 mg/mL, optimal concentration is 15 mg/mL). Centrifuge for 510 min at 14,00017,000 g at 4C in a microcentrifuge.

What is the detection range for western blot?

The western blot method is prescribed as the official Japanese confirmation test for egg proteins (see Table 17.3). These western blot kits use specific polyclonal antibodies against ovalbumin and ovomucoid. Their detection limit was determined to be 0.5 g/g of the allergenic ingredient per g of soluble protein.

What is western blotting limitation?

The main limitation of western blotting is that it can only be carried out if a primary antibody against the protein of interest is available. To detect post-translational modifications such as phosphorylation of target proteins, specific antibodies against the phosphorylated residues are needed.

How much protein is too much for western blot?

To obtain linear signals with the majority of western blots, we recommend loading smaller amounts of protein sample between 1 and 10 g per well. To avoid under- or overloading samples, determine the protein concentration of each sample prior to electrophoresis with a compatible protein assay.

What is the ideal cell number for a western blot?

it depends on the cell concentration of the proteins that you are looking for. As example for ubiquitous protein like actin or tubulin also 10*10e4 is well enought to run multiple WB. For less expressed protein, 1*10e6 should fit well.

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