Many people find the process to blot flag in WRF quite challenging, particularly if they don't regularly work with documents. Nevertheless, today, you no longer have to suffer through long guides or wait hours for the editing app to install. DocHub enables you to adjust documents on their web browser without installing new programs. What's more, our powerful service offers a full set of tools for comprehensive document management, unlike so many other online tools. That’s right. You no longer have to donwload and re-upload your forms so frequently - you can do it all in one go!
No matter what type of paperwork you need to modify, the process is straightforward. Take advantage of our professional online solution with DocHub!
Welcome to the Novus Visual Protocol Series. In this video we will learn how to perform all phases of a Western Blot using the most common methods for this assay. Before we can start preparing the blot we must first prepare our sample lysate. In this example we will prepare a protein lysate from cultured cells. Here we wash the cells twice with ice cold PBS and enough lysis buffer to cover the cells. The choice of lysis buffer depends largely upon the localization of your protein of interest. We scrape the cells and transfer the cell solution on a centrifuge tube placed on ice. In order to solubilize membrane bound proteins, we will require stronger extraction detergents compared to isolated cytoplasmic proteins. In this example we are using a standard RIPA buffer, which is a common buffer for obtaining maximum protein yield. While extracting proteins from all cellular localizations, it is very important to include protease inhibitors in your lysis buffer which will prevent degradation
